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malP [2018-07-26 13:04:20]
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malP [2018-07-26 13:04:20]

maltose-specific phosphotransferase system, EIICB of the PTS
locus
BSU08200
pI
8.87
mw
57.89 kDa
protein length
527 aa Sequence Blast
gene length
1581 bp Sequence Blast
function
maltose uptake and phosphorylation
product
maltose-specific phosphotransferase system, EIICB
essential
no
ec
2.7.1.69
synonyms
glvC
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.2|Phosphotransferase system] → [category|SW 1.2.2.2|Sugar specific PTS proteins]
  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.22|Utilization of maltose]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.3|Phosphorylation on a Cys residue]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.4|Phosphorylation on a His residue]

    • Gene

    Coordinates
    892,215 → 893,798

    Phenotypes of a mutant

  • no growth on maltose as single carbon source [Pubmed|10627040]

    • The protein

    Catalyzed reaction/ biological activity

  • Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate (according to Swiss-Prot)

    • Protein family

  • [protein|14ED1AF5038F43F3B151FCBABE6CFC5A2DA3AA6E|PtsI] permease, glucose permease (Glc) family [Pubmed|10627040]

    • [SW|Localization]

  • cell membrane (according to Swiss-Prot)

    • Expression and Regulation

    Operons

    genes
    [gene|C650160C9E5B5F8587CA9E80A57BCCE97D701037|malA]-[gene|AD72489DA260F07B97A213F1F0FBA7CED9471817|glvR]-[gene|E6C7733A12AF9A5322EED444E4A4FF1605A8B20F|malP]
    description
    [Pubmed|11489864]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|11489864], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|AD72489DA260F07B97A213F1F0FBA7CED9471817|GlvR]: activation, [Pubmed|11489864], in [regulon|AD72489DA260F07B97A213F1F0FBA7CED9471817|GlvR regulon]
  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|11489864], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]

    • regulation

  • induction by maltose ([protein|search|GlvR]) [Pubmed|11489864]
    • view in new tab

    Biological materials

    Mutant

  • MGNA-C291 (glvC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2289 NBRP B. subtilis, Japan]
  • GP110 (spc), available in [SW|Stülke] lab
  • BKE08200 (Δ[gene|E6C7733A12AF9A5322EED444E4A4FF1605A8B20F|malP]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE08200 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTGCATCATACCAGAACCCC, downstream forward: _UP4_TAAAGACTGCCCTCCTTTTC
  • BKK08200 (Δ[gene|E6C7733A12AF9A5322EED444E4A4FF1605A8B20F|malP]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK08200 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTGCATCATACCAGAACCCC, downstream forward: _UP4_TAAAGACTGCCCTCCTTTTC

    • References

  • 16707683,10627040,11489864